This field is for entering in the name of the species for which information is being reported. Please use the full binomial (e.g., Capsicum annuum) for each entry, without the authority, subspecies, or population information. If this information needs to be included, it may be included as a note appended to the species field (see species note below).

Species notes could refer to specific populations, if population structure is important for understanding the applicability of the information presented.

General instructions for notes:

This field, and all others like it, are for adding in a number which will be used as a superscript to relate a note for a field to the text of the note.

The text for all notes for an entry is entered into one field below. Notes should be used sparingly to add in information which will be generally important to researchers in the field.

The numbers in note fields should be sequential down the form, 1–N, as they are used.

This field is for entering in the Family name of the species.

This note may be used for attaching information to the family field. General instructions for notes.

This field is for the locus name. Please limit names to Roman alphanumeric characters (a–z, A–Z, 0–9, and hyphens). If possible, please try to make locus names as unique as possible (you may search the database for locus names you are considering to see if there is anything similar already).

This note may be used for attaching information to the locus field. General instructions for notes.

Please select a marker type from the drop-down list. Single nucleotide polymorphisms (SNP) includes all cloned genes where sequence comparisons are made, either by sequencing or allele-specific amplification. Cleaved (or cut) amplified polymorphic sequences (CAPS) are PCR products that have been restricted to reveal polymorphism; the enzyme used should be indicated in the marker type note field.

This list may be expanded in the future to accomodate new marker types.

This note may be used for attaching information to the marker type field. General instructions for notes.

This field is for describing the repeat motif for cloned microsatellite alleles, and may be ignored for other types of markers or other species for which only amplification information is known.

When filling out this field, you should indicate the number of repeats by subscripting, which is accomplished by bracketing the number or symbol to be subscripted with the html subscript tags:
₁₄.

This note may be used for adding information about the repeat motif. General instructions for notes.

Select the genomic compartment of the marker from the drop-down list. Ribosomal DNA is allowed as a special subset of markers.

This note may be used to clarify the genome field. General instructions for notes.

Enter in the database ID of the original cloning record.

Enter in the database name where the accession was submitted.

This field is for indicating whether this record is from the species from which the locus was originally derived. If so, enter a Y in the field; if not, enter in the full name of the species from which the locus was derived.

For example, if I were reporting amplification of a microsatellite in Capsicum chinense that was oringinally cloned from Capscium annuum, I would enter Capsicum annuum in the field.

The utility of this field presumes that the record for the original species also is/will be in the database.

This field is for entering in the forward primer for the locus. Please enter the sequence in capitals in the 5'–3' direction.

This field is for attaching additional information about the primer sequence. General instructions for notes.

This field is for entering in the reverse primer for the locus. Please enter the sequence in capitals in the 5'–3' direction.

This field is for attaching additional information about the primer sequence. General instructions for notes.

This field is for entering in the PCR annealing temperature used during amplification. A °C will automatically be attached.

This field is for attaching information about the annealing temperature, which may include details about cycling conditions related to annealing temperature.

This field describes the number of alleles observed in assays of the species or group described by this record.

If amplification was attempted for group, but was unsuccessful, enter a 0 in this box and the record will read, "tried, but no amplification."

This field may be used to attach information to the number of alleles, and could be used to relay information about the presence of null alleles or the size of population tested. General instructions for notes.

This field provides the size range of the detected alleles. Range may be indicated by either a hyphen or the html code for an en dash (–) as in the example. The latter provides this output: 155–165.

This field may be used to attach information about the size range. General instructions for notes.

This field is for entering in the expected heterozygosity if the size of population screened was adequate to calculate H_e.

This field may be used to attach information to the H_e measure. General instructions for notes.

This field is for entering in the observed heterozygosity if the size of population screened was adequate to measure H_o.

This field may be used to attach information to the H_o measure. General instructions for notes.

This field is for the contact authors name.

This field is for the contact authors email. If no email address is available please enter a fax number by typing in "fax: number".

This area is for entering in the text for the note fields given numbers above. This may be accomplished most easily by writing the note in a text processor, and then pasting the information in this field.

The text for each note should have the number, a period, and then the note text, followed by the characters <p> which are the equivalent of a line break.

If no notes are needed for an entry, please be sure to delete the text in this field before submitting the record to the database.